diff --git a/zuul.d/rally.yaml b/zuul.d/rally.yaml
index 94b4bf0a7f1..e04778369ee 100644
--- a/zuul.d/rally.yaml
+++ b/zuul.d/rally.yaml
@@ -4,7 +4,6 @@
     timeout: 10800
     vars:
       devstack_localrc:
-        USE_PYTHON3: true
         OSPROFILER_COLLECTOR: redis
         OSPROFILER_HMAC_KEYS: "neutron-hmac-key-used-in-zuul-ci"
         Q_ML2_TENANT_NETWORK_TYPE: vxlan
@@ -180,7 +179,6 @@
         # is finished or the mech driver does not call monkey_patch().
         Q_ML2_PLUGIN_MECHANISM_DRIVERS: ovn
         Q_ML2_TENANT_NETWORK_TYPE: geneve
-        USE_PYTHON3: true
         PHYSICAL_NETWORK: public
         Q_USE_PROVIDERNET_FOR_PUBLIC: true
         ENABLE_CHASSIS_AS_GW: true
diff --git a/zuul.d/tempest-multinode.yaml b/zuul.d/tempest-multinode.yaml
index 35aae7025be..29bbcf73863 100644
--- a/zuul.d/tempest-multinode.yaml
+++ b/zuul.d/tempest-multinode.yaml
@@ -536,7 +536,6 @@
           PHYSICAL_NETWORK: public
           ENABLE_CHASSIS_AS_GW: false
           OVN_DBS_LOG_LEVEL: dbg
-          USE_PYTHON3: True
           ENABLE_TLS: True
 
 - job:
diff --git a/zuul.d/tempest-singlenode.yaml b/zuul.d/tempest-singlenode.yaml
index 87076d90868..1d43dad579c 100644
--- a/zuul.d/tempest-singlenode.yaml
+++ b/zuul.d/tempest-singlenode.yaml
@@ -498,7 +498,6 @@
         ENABLE_CHASSIS_AS_GW: true
         OVN_L3_CREATE_PUBLIC_NETWORK: true
         OVN_DBS_LOG_LEVEL: dbg
-        USE_PYTHON3: True
         BUILD_TIMEOUT: 784
         ENABLE_TLS: True
         OVN_IGMP_SNOOPING_ENABLE: True